The remedy outcomes of this group of individuals had been also in contrast

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 The remedy outcomes of this group of individuals had been also in contrast  Empty The remedy outcomes of this group of individuals had been also in contrast

Post  wangqian on Wed May 07, 2014 5:31 am

SKGT4 cells were stimulated with 300 ìM DCA or 300 ìM ursodeoxycholic acid for 06 hr. We now have previ ously shown that UDCA, in contrast to DCA, doesn't induce AP 1 transcription aspect activation in colon cancer cells. Actually, it inhibits abt263 費用 interleukin 1 beta and deoxycholic acid induced activation of NF kappaB and AP 1 in these cells. Cell proliferation was assessed employing the MTT assay. DCA induces a dose and time dependent lessen in cellular proliferation, that is at first observed inside of the very first hour of treatment, remains at similar levels up to 8 hr and it is much more pronounced at 12 and 24 hr. This decrease is clear at 300 ìM DCA and greater concen trations, staying statistically considerable at 400500 ìM.

Dramatic morphological changes indicative of apoptosis are also observed at 6 hr of DCA therapy at concentrations in extra of 300 ìM. In comparison, cells stimulated with UDCA demonstrate identical proliferation patterns and morphology as in contrast Adriamycin 臨床試験 to untreated cells in any way occasions and concentra tions tested. DNA fragmentation and PARP cleavage, two on the hall marks of apoptosis, had been respectively assessed by quanti fying cytoplasmic histone associated DNA fragments by ELISA and Western blotting utilizing a certain antibody that recognizes the 85 kDa cleaved PARP fragment. DCA dose response and kinetic studies showed that a low amount of PARP cleavage was detected at 6 hr submit DCA treatment and persisted for up to 24 hr. This effect is dose dependent, remaining observed at 300500 ìM DCA but not at reduced concentrations.

Similarly, DCA induced a 1. 5 fold boost in DNA fragmentation after 6 hr, which elevated immediately supplier ABT-199 after 24 hr. DNA fragmen tation with very low concentrations of DCA was much like resting cells, when raising concentra tions resulted in a regular rise in DNA injury. Taken together, these data demonstrate that DCA induces a reduction in cell proliferation, which can be accompanied by very low amounts of apoptosis. These results are sustained and dose dependent, becoming observed at high DCA concentrations much like individuals found in patients with erosive esophagitis and Barretts esophagus. DCA induced PARP cleavage is caspase dependent PARP cleavage can arise through caspase dependent and inde pendent mechanisms.

The broad spectrum caspase inhibitor, Z Val Ala Asp CH2F, and the distinct caspase 3 inhibitor, Z Asp Glu Val Asp FMK, had been employed to assess the role of caspases in DCA induced PARP cleavage. SKGT4 cells had been pretreated for 1 hr with 50 ìM of either Z VAD FMK or Z DEVD FMK and stimulated with 400 ìM DCA, a concentration which induced substantial ranges of PARP cleavage for 6 hr. Unstimulated SKGT4 cells showed negligible levels of PARP cleavage and DNA fragmentation. The two Z VAD FMK and Z DEVD FMK com pletely abolished DCA induced PARP cleavage even though par tially inhibiting DNA fragmentation. These information indicate that DCA induced PARP cleavage is caspase 3 dependent, while DNA fragmentation is only partially dependent on this pathway. DCA induces COX 2 expression by means of Erk12 and p38 dependent mechanisms Interestingly, the ranges of DCA induced PARP cleavage plateau and don't boost progressively. This suggests that a compensatory survival mechanism might be con comitantly regulated by DCA.


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