In view of the unclear etiol ogy of asthma, the purpose of asthma treatment is
-=[ Braila.Bossforum.net ]=- :: Connect 85.121.207.195:27016 ip serverului de Half-Life :: Cereri ADMINE & SLOT [adminul se da in maxim 1h & Slotul pe loc]
Page 1 of 1
In view of the unclear etiol ogy of asthma, the purpose of asthma treatment is
The peripheral effects of neuro tensin appear to be mediated largely by NTSR1, which activates PLCb. Experiments using a specific antagonist or knockdown of the NTSR1 using short interfering RNA suggest that NTSR1 mediates the effects of neurotensin on cancer cells, although NTSR3 sortilin, which is often coexpressed in cancer cells, may modulate INNO-406 溶解度 NTSR1 signalling. Splice variants of the NTSR1 were recently detected in prostate cancer cell lines, however, no functional studies of these have been conducted. Recent data have suggested that the NTSR1 receptor gene may be a downstream target of the extracellular signal regulated kinase and Tcf b catenin pathways, and increased expres sion of NTSR1 during progression of colon tumorigen esis has been reported.
Neurotensin has been found to stimulate proliferation of certain colon carcinoma cell lines. Reports on intracellular signalling leading to proliferation induced by neurotensin in some Lapatinib 分子量 other cell types have suggested the involvement of PKC dependent activation of ERK and protein kinase D, and either dependence or independence of epidermal growth factor receptor transactivation. In the pancrea tic cancer cell line Panc 1, DNA synthesis induced by neurotensin was independent of EGFR transactivation, whereas in the prostate cancer cell line PC 3, neu rotensin stimulated mitogenesis by a PKC dependent transactivation of EGFR. In colon carcinoma cell lines neurotensin has been found to activate ERK, as well as PKC, Akt, and nuclear factor B path ways.
Furthermore, neurotensin induced LY2109761 700874-71-1 phos phorylation and inactivation of glycogen synthase kinase, leading to cyclin D1 expression, through mechanisms that were at least partly dependent on PKC. Neurotensin has also been found to induce a proinflammatory tumour microenvironment and pro mote cancer cell invasion through pathways that involved NF B, PKC, ERK, and the sodium proton exchanger 1. The aim of the present study was to investigate some of the intracellular signalling pathways involved in mito genesis induced by neurotensin in human colorectal cancer cells, by examining the HCT116 and HT29 lines and comparing them with Panc 1 cells. The results sug gested that while neurotensin acted predominantly through PKC in Panc 1 cells and via EGFR transactiva tion in HT29 cells, it used both these pathways in HCT116 cells.
In the latter cells neurotensin induced activation of ERK was mediated largely by PKC, while neurotensin induced activation of Akt was independent of PKC but involved transactivation of the EGFR, appar ently by a Ca2 dependent mechanism. Neurotensin induced DNA synthesis was mediated mainly by PKC. Methods Chemicals Dulbeccos modified Eagles medium, N piperazine N, penicillin and streptomycin were from Gibco. Neurotensin, 12 O tetradecanoylphorbol 13 acetate, thapsigargin, epidermal growth factor, and wortmannin were obtained from Sigma Aldrich. maleimide], 4 6,7 dimethoxyquinazoline, 2 amino 3 methoxyflavone 2 4 methylpentanoyl] L tryptophan methylamide were from Calbiochem. 7 Methyl 2 9 4H pyrido pyrimidin 4 one was obtained from Cayman Chemical. Transforming growth factor a was obtained from Bachem.
Neurotensin has been found to stimulate proliferation of certain colon carcinoma cell lines. Reports on intracellular signalling leading to proliferation induced by neurotensin in some Lapatinib 分子量 other cell types have suggested the involvement of PKC dependent activation of ERK and protein kinase D, and either dependence or independence of epidermal growth factor receptor transactivation. In the pancrea tic cancer cell line Panc 1, DNA synthesis induced by neurotensin was independent of EGFR transactivation, whereas in the prostate cancer cell line PC 3, neu rotensin stimulated mitogenesis by a PKC dependent transactivation of EGFR. In colon carcinoma cell lines neurotensin has been found to activate ERK, as well as PKC, Akt, and nuclear factor B path ways.
Furthermore, neurotensin induced LY2109761 700874-71-1 phos phorylation and inactivation of glycogen synthase kinase, leading to cyclin D1 expression, through mechanisms that were at least partly dependent on PKC. Neurotensin has also been found to induce a proinflammatory tumour microenvironment and pro mote cancer cell invasion through pathways that involved NF B, PKC, ERK, and the sodium proton exchanger 1. The aim of the present study was to investigate some of the intracellular signalling pathways involved in mito genesis induced by neurotensin in human colorectal cancer cells, by examining the HCT116 and HT29 lines and comparing them with Panc 1 cells. The results sug gested that while neurotensin acted predominantly through PKC in Panc 1 cells and via EGFR transactiva tion in HT29 cells, it used both these pathways in HCT116 cells.
In the latter cells neurotensin induced activation of ERK was mediated largely by PKC, while neurotensin induced activation of Akt was independent of PKC but involved transactivation of the EGFR, appar ently by a Ca2 dependent mechanism. Neurotensin induced DNA synthesis was mediated mainly by PKC. Methods Chemicals Dulbeccos modified Eagles medium, N piperazine N, penicillin and streptomycin were from Gibco. Neurotensin, 12 O tetradecanoylphorbol 13 acetate, thapsigargin, epidermal growth factor, and wortmannin were obtained from Sigma Aldrich. maleimide], 4 6,7 dimethoxyquinazoline, 2 amino 3 methoxyflavone 2 4 methylpentanoyl] L tryptophan methylamide were from Calbiochem. 7 Methyl 2 9 4H pyrido pyrimidin 4 one was obtained from Cayman Chemical. Transforming growth factor a was obtained from Bachem.
jy9202- Posts : 509
Join date : 2013-12-18
Similar topics
» How Do Chemical Peels Purpose To Eradicate Zits Scars?
» but would seem to support the purpose of docetaxel in inhibit ing tubulin polym
» Chemistry Study Help Illustrate An Essential Purpose of Pupils
» Think view to get FIFA coins
» Irrespective of the duration of treatment, both CBHA and TSA responsive
» but would seem to support the purpose of docetaxel in inhibit ing tubulin polym
» Chemistry Study Help Illustrate An Essential Purpose of Pupils
» Think view to get FIFA coins
» Irrespective of the duration of treatment, both CBHA and TSA responsive
-=[ Braila.Bossforum.net ]=- :: Connect 85.121.207.195:27016 ip serverului de Half-Life :: Cereri ADMINE & SLOT [adminul se da in maxim 1h & Slotul pe loc]
Page 1 of 1
Permissions in this forum:
You cannot reply to topics in this forum
|
|